The PETRATECHvitro platform labeled "Plateforme Aix-Marseille" is specialized in the production of preclinical in vitro 3D models of primary brain tumors and brain metastases, adults and pediatrics, derived from samples of surgical excisions (surgical waste) of patients operated in the Departments of neurosurgery at the APHM and transmitted by the Biological Resource Center of the APHM, within the framework of regulated protocols (CODECOH Declaration File DC-2025-7312).
We use and analyze these models in the evaluation of response to treatments and neurotoxicity.


Spheroids of human glioblastoma cancer stem cell primary cell lines with different molecular profiles.
Tumoroids and explants derived from patient tumors: IDH-wt glioblastomas, meningiomas, other adult or pediatric primary brain tumors, brain metastases – lung, colon, breast, other.
Production of 3D models: spheroids, tumoroids and explants
Cryopreservation and paraffin inclusion of models
Time-lapse video microscopy
Evaluation of response to treatment and neurotoxicity
Histopathological analyses
Additional immunostaining analyses
Advice and training
L2 Laboratory
Biological Safety Cabinets
Tissue Chopper
CO2 Incubator
Orbital shaker for incubator
Celloger Pro Video Microscope
Inverted fluorescence microscope
HALO 4K Digital Microscope
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